Recombinant Histone 3 antibody (H3K36me3)
Quick Overview for Recombinant Histone 3 antibody (H3K36me3) (ABIN6731010)
Target
See all Histone 3 (H3) AntibodiesAntibody Type
Reactivity
Host
Clonality
Conjugate
Application
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Binding Specificity
- H3K36me3
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Brand
- AbFlex®
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Purification
- Protein A Chromatography
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Immunogen
- This antibody was raised against a peptide containing trimethly-lysine 36 of histone H3.
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Isotype
- IgG2a
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Application Notes
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Validated Applications:
< strong style="margin-left:50px">ChIP-Seq: 4 μg per ChIP
< strong style="margin-left:50px">WB: 0.5 - 2 μg / mL
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Comment
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AbFlex® antibodies are recombinant antibodies (rAbs) that have been generated using defined DNA sequences to produce highly specific, reproducible antibodies. Each AbFlex antibody contains a 6XHis tag, an avidin tag sequence for enzymatic biotin conjugation using the biotin ligase, BirA, and a sortase recognition motif (LPXTG) to attach a variety of labels directly to the antibody including fluorophores, enzymatic substrates (HRP, AP), peptides, drugs as well as solid supports. AbFlex H3K36me3 antibody was expressed as full-length IgG with mouse immunoglobulin heavy and light chains (IgG2a isotype) in mammalian 293 cells.
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Restrictions
- For Research Use only
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Format
- Liquid
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Buffer
- Purified IgG in 140 mM Hepes, pH 7.5, 70 mM NaCl, 32 mM NaOAc, 0.035 % sodium azide, 30 % glycerol.
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Preservative
- Sodium azide
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Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Storage
- -20 °C
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- Histone 3 (H3) (Histone H3 (H3))
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Alternative Name
- Histone H3
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Molecular Weight
- 17 kDa
Target
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